In this chapter we focus on genetic recombination and chromosome mapping. Complex processes have evolved in bacteria and bacteriophages that facilitate genetic recombination within populations. As we shall see, these processes are the basis for the chromosome mapping analysis that forms the cornerstone of molecular genetic investigations of bacteria and the viruses that invade them.
- 9.1 Bacteria Mutate Spontaneously and Grow at an Exponential Rate
- 9.2 Conjugation Is One Means of Genetic Recombination in Bacteria
- F+ and F- Bacteria
- Hfr Bacteria and Chromosome Mapping
- Recombination in F+ X F- Matings: A Reexamination
- The F’ State and Merozygotes
- 9.3 Rec Proteins Are Essential to Bacterial Recombination
- 9.4 F Factors Are Plasmids
- 9.5 Transformation Is Another Process Leading to Genetic Recombination in Bacteria
- Transformation and Linked Genes
- 9.6 Bacteriophages Are Bacterial Viruses
- Phage T4: Structure and Life Cycle
- The Plaque Assay
- Lysogeny
- 9.7 Transduction Is Virus-mediated Bacterial DNA Transfer
- The Lederberg–Zinder Experiment
- The Nature of Transduction
- Transduction and Mapping
- 9.8 Bacteriophages Undergo Intergenic Recombination
- Bacteriophage Mutations
- Intergenic Mapping
