Biological Science

Chapter 16: Genomes

Summary Review

1. What is the primary innovation that has led to the current explosion in the field of "genomics" (as opposed to the slower pace of this study in the past)? [Hint]

2. A bacteria, Vibrio cholera, causes diarrhea and severe disease in people worldwide. Which of the following would be the MOST LIKELY reason for sequencing the genome of this bacteria? [Hint]

3. According to this figure, which of the following is a true statement?
16m1q3.jpg [Hint]

4. If you have an organism with 2 million base pairs, which of the following could be the first step that you use to sequence its genome? [Hint]

5. What enzyme is most critical to the sequencing reaction shown here? [Hint]

6. What type of problem would most likely reduce the accuracy of the sequencing strategy shown? [Hint]

7. What is the effect of large numbers of regions of AT repeats on sequencing a genome? [Hint]

8. Why are mRNA sequences generally used in the study of genome sequences in eukaryotes? [Hint]

9. This figure shows the process of creating cDNA from an mRNA transcript. What enzyme is involved in synthesizing cDNA from mRNA?
16m1q9.jpg [Hint]

10. Which of the following statements is NOT true? [Hint]

11. Which of the following would NOT be considered an open reading frame (ORF)? [Hint]

12. Scientists studying the genomes of “normal,” benign E. coli and the pathogenic E. coli strain O157:H7 have made which of the following determinations? [Hint]

13. If a bacteria were to develop resistance to an antibiotic, which of the following methods would most likely result in the development of antibiotic resistance in other species of bacteria that are located in close proximity to the resistant bacteria? [Hint]

14. Which of the following is NOT a major source of genetic variation in bacteria? [Hint]

15. Transposable elements [Hint]

16. If there were a mutation in the reverse transcriptase formed in this cell that prevented it from binding to the LINE mRNA, how would the resulting situation differ from the diagram shown here?

16m1q16.jpg [Hint]

17. This figure demonstrates the way that the number of repeating units can be changed during the production of gametes. In comparison of the pattern of repeat sequences in dizygotic (non-identical) twin brothers, what would you expect to find?

16m1q17.jpg [Hint]

18. The globin gene family shown is thought to have arisen from which of the following processes?

16m1q18.jpg [Hint]

19. Assume that you are a researcher who is looking to see which of the sequences in this microarray may contain the gene that codes for the production of a specific protein hormone in the human ovary. How would you produce the labeled cDNA’s used to probe the microarray? [Hint]

20. Which of the following may explain why, even if a mouse develops an immune response to a given protein, it may still not serve as a useful vaccine?

16m1q20.jpg [Hint]

Answer choices in this exercise are randomized and will appear in a different order each time the page is loaded.
 




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