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| 1. |
Explain the relationship between promoter sequences, transcription factors, and RNA polymerase.
To create paragraphs in your essay response, type <p> at the beginning of the paragraph, and </p> at the end.
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| 2. |
According to the wobble rules, the correct amino acid can be added to a growing polypeptide chain even if the third base in an mRNA codon does not correctly match the corresponding base in a tRNA anticodon. How do the wobble rules and the redundancy of the genetic code relate?
To create paragraphs in your essay response, type <p> at the beginning of the paragraph, and </p> at the end.
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| 3. |
Why does splicing occur in eukaryotic mRNAs? Where does it occur, and how are snRNPs involved?
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| 4. |
An in vitro RNA synthesis reaction was set up and allowed to proceed in the presence of nonradioactive ribonucleotides (NTPs). After several minutes had passed, radioactive NTPs were added and RNA synthesis was allowed to continue. Then the RNA molecules were isolated from the reaction mixture and analyzed for the presence of radioactive nucleotides at the 5' and the 3' ends. Based on what you know about RNA synthesis, which end of the RNA molecules were found to be radioactive? (Assume that conditions were set so that each ribosome transcribed a single message.)
To create paragraphs in your essay response, type <p> at the beginning of the paragraph, and </p> at the end.
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| 5. |
Sketch the structure of a tRNA. Label the anticodon and the CCA sequence where amino acids bind. Explain how a tRNA interacts with tRNA synthetase. Sketch the structure of an aminoacyl tRNA. Explain how an aminoacyl tRNA interacts with an mRNA in the ribosome.
To create paragraphs in your essay response, type <p> at the beginning of the paragraph, and </p> at the end.
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| 6. |
How did the A-site, P-site, and E-site of the ribosome get their names? Explain the sequence of events that occurs during translation as a protein elongates by one amino acid.
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| 7. |
What evidence supports the hypothesis that peptide bond formation is catalyzed by a ribozyme?
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