Biological Science

Chapter 5: Activities

Activity 5.2 Pulse-Chase Experiment

CDA5_2.jpg A pulse-chase experiment allows an investigator to track the fates of proteins through a cell, from the protein's synthesis to its final cellular destination. In this activity, you'll explore a pulse-chase experiment at the molecular and cellular levels. The experiment is based on those performed by George Palade and colleagues, who determined the roles of the rough endoplasmic reticulum and Golgi apparatus in the secretion of proteins.

Molecular View of the Pulse-Chase Experiment
Cellular View of the Pulse-Chase Experiment
Tracking the Radioactivity

Chapter 5, Section 5.3, The Endomembrane System: Synthesis and Distribution of Cellular Products





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